Hybrid maize seed production in Africa can face reduced yields based on the damage that may be incurred when detasseling female plants. Moreover, incomplete detasseling may lead to impurities hybrid seed production. A unique nuclear genetic male sterility seed production technology, referred to as Ms44-SPT, was developed to reduce these hybrid seed production challenges. Data collected to assess the impact of the Ms44-SPT technology on leaf loss during detasseling and on seed production are reported in this dataset.