This dataset is derived from a study focused on developing an efficient method for arabinoxylan quantification, called PentoQuant. It includes phenotypic and molecular characterization data from 606 bread wheat samples developed through the spring bread wheat breeding program. The dataset comprises total and water-extractable arabinoxylan content values measured using the PentoQuant protocol. Furthermore, it incorporates results obtained from analyzing the same 606 lines with four molecular markers associated with two major QTLs for arabinoxylan content variation in wheat, located on chromosomes 1B and 6B.
