A study was conducted to evaluate Trifolium species for their agronomic traits in two agro-ecologies of Benishangul-Gumuz region, North Western Ethiopia. The experiment was carried out at Tongo, Assosa, and Kamash forage research stations of Assosa Agricultural Research Center, which were purposively selected to represent highland, mid and lowland agro-ecologies respectively. Five Trifolium genotypes (T. steudeneri 9720, T. decorum 9447, T. quantinum 6301, T. tembense 7102, and T. steudeneri 9712) were evaluated in a randomized complete block design with three replications. Plant height at forage harvesting, forage dry matter yield and leaf to stem ratio were significantly (P < 0.001) affected by environment (E) and G x E (G and E interaction). The highest mean forage dry matter yield was recorded at Kamash, than Assosa and Tongo. The result of combined analysis indicated that, genotype (G) had significant (P < 0.001) effect on forage dry matter yield and T. quantinum 6301 genotype was the best yielder among the genotypes evaluated. Total dry matter yield was significantly different (P < 0.001) among genotypes at Tongo and Assosa. Generally, based on forage dry matter yield data, T. quantinum 6301 was well performed at Assosa and Tongo than other genotypes, T. steudenary 9720 was relatively better adapted at Kamash, and they are recommended as an alternative legume forage crops for the respective locations.
